Fig. 1

Costunolide inhibited the viability of gastric adenocarcinoma BGC-823 cells. a Chemical structure of costunolide. Molecular formula: C₁₅H₂₀O₂, molecular weight: 232.32, CAS No.: 553–21-9. b Purity of costunolide detected by HPLC. The purity is 99.87%. c The viability of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20, 25, 30, 35 and 40 μM) for 24 and 48 h is detected by MTT assay. d The morphology of BGC-823 cells treated by costunolide with different concentration (0, 5, 10, 15, 20 and 25 μM) for 24 and 48 h is observed by light inverted microscopy (magnification: 100×, bar in the picture represents 100 μm). Compared to control group, *P < 0.05, **P < 0.01