Table 1 The gradient eluting pattern for cucurbitacin B determination by using LC-MS/MS.
From: Pharmacokinetics of cucurbitacin B from Trichosanthes cucumerina L. in rats
| Time (min) | Methanol (%) | 0.2% v/v Formic acid (%) |
|---|---|---|
| 0.00 | 45 | 55 |
| 2.50 | 90 | 10 |
| 3.00 | 90 | 10 |
| 3.50 | 45 | 55 |
| 5.00 | 45 | 55 |