Table 1 The gradient eluting pattern for cucurbitacin B determination by using LC-MS/MS.
From: Pharmacokinetics of cucurbitacin B from Trichosanthes cucumerina L. in rats
Time (min) | Methanol (%) | 0.2% v/v Formic acid (%) |
|---|---|---|
0.00 | 45 | 55 |
2.50 | 90 | 10 |
3.00 | 90 | 10 |
3.50 | 45 | 55 |
5.00 | 45 | 55 |