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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: In vitro anti-nephrotoxic potential of Ammi visnaga, Petroselinum crispum, Hordeum vulgare, and Cymbopogon schoenanthus seed or leaf extracts by suppressing the necrotic mediators, oxidative stress and inflammation

Fig. 5

Microscopic investigation and flow cytometric analysis for the carbon tetrachloride (CCl4)-induced necrosis in Vero Cells and the anti-necrotic effect of the studied extracts in comparison with the Ketosteril (Ks). (a) Photomicrographs taken by the inverted microscope (b) Heat map distribution of the necrotic mediators, the color distributed from white (low level/expression or activity) to blue (high level/expression or activity) (c) Nuclear double staining of Vero cells using acridine orange (AO)/ethidium bromide (EB) (d) Annexin V/Propidium Iodide (PI) flow cytometry charts (e) Quantification of the necrotic cell populations, results are presented as mean ± SE (n = 3). Different letters for the same parameter are significantly different at p < 0.05. C; the untreated control cells, AVE, Ammi visnaga extract; PCE, Petroselinum crispum extract; HVE, Hordeum vulgare extract; CSE, Cymbopogon schoenanthus extract; VC, viable cells; EN, Early necrotic cells; LN, Late necrotic cells

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BMC Complementary Medicine and Therapies

ISSN: 2662-7671

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