Fig. 4

The effects of SZC on the overall structure of gut microbiota in mice with CID (n = 5, male/femal = 2–3). (a) Species accumulation boxplot was used for the estimations of samples number and species richness. (b) Venn diagram indicated the overlap of OTUs in the feces. (c) OTUs were clustered from high-quality sequences with 97% similarity. (d–e) ACE and Shannon values indicated the bacterial community richness and diversity, respectively. (f) NMDS plot showed distinct structural changes of the overall bacterial community in each group. (g) UPGMA clustering was used to interpret the distance matrix of each sample by the average linkage. Data were presented as means ± SEM. ^*P < 0.05 and ^**P < 0.01 compared with model, ^#P < 0.05 and ^##P < 0.01 compared with control