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Fig. 2 | BMC Complementary and Alternative Medicine

Fig. 2

From: Shen Shuai IIRecipe attenuates renal injury and fibrosis in chronic kidney disease by regulating NLRP3 inflammasome and Sirt1/Smad3 deacetylation pathway

Fig. 2

SSR inhibited NLRP3 inflammasome activation and IL-1βsecretion in 5/6(A/I) rats. (a) The protein expression of NLRP3,ASC, Pro-caspase1 and Caspase-1 p12 was determined by immunoblotting. (b) Western blot quantification of NLRP3, ASC, and Caspase-1 p12 levels. (n = 6) (c) The protein levels of Pro-IL1β and IL1βwere determined by immunoblotting. (d) The ratio of IL-1βto GAPDH protein was determined. (n = 6) (e) Representative images of IF staining for α-SMA and F4/80. Original magnification,× 400. Values are mean ± SD. *P < 0.05, **P < 0.01

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