Fig. 5

Effects of bioconverted mulberry leaf extract (BMLE) and unaltered mulberry leaf extract (MLE) on oral glucose tolerance test (OGTT) and insulin tolerance test (ITT) in obese diabetic mice. MLE (300 and 600 mg/kg) or BMLE (300 and 600 mg/kg) were administered (PO) for 7 weeks in obese diabetic mice. Blood was collected from the tail vein (a) 15, 30, 45, 60, and 120 min after oral administration of glucose (2 g/kg) for the OGTT and (b) 30, 45, 60, and 120 min after administration (SC) of insulin (1 U/kg) for the ITT (see Materials and Methods). Metformin (300 mg/kg) was used as a positive control. Data are expressed as the mean ± SEM (n = 7), and significant changes among experimental groups were interpreted by converting all data into the area under the curve (AUC). ** P < 0.01, versus high-fat diet-fed (HFD) diabetic mice control (vehicle treatment), and ^# P < 0.05, vs. MLE