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Table 6 In vitro chromosome aberration test in chinese hamster lung cells with OE extract from cactus stem

From: An oral toxicity test in rats and a genotoxicity study of extracts from the stems of Opuntia ficus-indica var. saboten

Treatment Schedulea Dose (μg/mL) Ratio of Aberrant Metaphaseb Cell Countsc Mean RICC(%)d
6–18 (+S) 0   0 4792 4689 4741 100
500   0 5024 4931 4978 108
1500 P 0 4996 4774 4885 105
3000 P 2 4405 4247 4326 86
3500 P 2 4158 4107 4133 80
4000 P 1 3528 3491 3510 59
4500 TTP 1 3054 2971 3013 42
5000 TTP 2 2796 2756 2776 34
B[a]P 20   30 3066 3116 3091 45
6–18 (-S) 0   0 5899 5757 5828 100
500   0 5525 5389 5457 91
1500 P 1 5001 4962 4982 79
1800 P 2 4243 4539 4391 65
2000 P 0 3508 3445 3477 42
3000 P 2 2942 2734 2838 26
4NQO 0.4   18 3226 3148 3187 35
24–0 (-S) 0   0 5600 5444 5522 100
500   1 5400 5479 5440 98
1500 P 1 4971 4971 4971 85
3000 P 1 4153 4072 4113 63
4000 P 0 3863 3842 3853 56
5000 TTP 0 3652 3670 3661 51
4NQO 0.4   21 2853 2994 2924 31
Initial cell count Cell countsc Mean
1754 1673 1872 1750 1762
  1. aTreatment time – Recovery time in the presence (+S) and absence (-S) of metabolic activation system
  2. bRatio of metaphase with chromosome aberrations. One culture/ dose was used
  3. Gaps excludes, 100 metaphases/ culture were examined
  4. cAfter harvesting mitotic cells, each culture was trypsinized and suspended with 0.5 mL of 0.1% trypsin and 5 mL of culture medium
  5. The cell suspensions of 0.4 mL per culture were diluted 50 times with 19.6 mL of Isoton sol. The cells in 0.5 mL Isoton sol. were counted twice/ culture using Coulter Counter model Z2
  6. Actual number of cells per flask = Mean Count × 550
  7. dRelative Increase in cell Count = ((Cell count of treated flask – Initial cell count) / (Cell count of the negative control flask – initial cell count)) × 100 (%)
  8. T: Turbidity at the end of the treatment
  9. P: Precipitation at the end of the treatment
  10. B[a]: benzo[a] pyrene, 4NQO: 4-Nitroquinoline-1-oxide