Table 1 PCR primers used for molecular identification of Candida species tested in this study
Molecular method | Species identified | Primer name | Sequence (5′ → 3′) | Amplicon size | Reference |
|---|---|---|---|---|---|
Singleplex PCR | C. albicans | CR-f | GCTACCACTTCAGAATCATCATC | ~ 960 bp | Romeo and Criseo, 2008 |
CR-r | GCACCTTCAGTCGTAGAGACG | ||||
Multiplex PCR | C. glabrata | UNI-5,8S | ACCAGAGGGCGCAATGTG | ~ 397 bp | Romeo et al., 2009 |
GLA-f | CGGTTGGTGGGTGTTCTGC | ||||
NIV-f | AGGGAGGAGTTTGTATCTTTCAAC | ||||
BRA-f | GGGACGGTAAGTCTCCCG | ||||
Multiplex PCR | C. parapsilosis | mCPF | TTTGCTTTGGTAGGCCTTCTA | ~ 171 bp | Asadzadeh et al., 2015 |
mCOF | TAAGTCAACTGATTAACTAAT | ||||
mCMF | AACTGCAATCCTTTTCTTTCTA | ||||
mLEF | TACAGAATTTTGAGAATTGTG | ||||
mCPCR | AATATCTGCAATTCATATTACT |