Fig. 4

Effects of a PKA inhibitor (H89) on PHME-stimulated lipolysis. a Fully differentiated 3T3-L1 adipocytes were treated with 200 μg/mL PHME alone or in the presence of 10 μM H89 for 12 h, then glycerol released into the medium was quantified. b Fully differentiated 3T3-L1 adipocytes were treated with 400 μg/mL PHME alone or in the presence of 10 μM H89 for 12 h, then cells were lysed, and then basal and phosphorylated levels of HSL were measured using western blotting analysis. Values are expressed as mean ± SD (n = 4), **P < 0.01 vs. control group