Fig. 3

Expression and phosphorylation of S6K1, mTOR, AMPK and HK2. SKOV3 cells were treated with cardamonin (5, 20 μM), rapamycin (0.1 μM) and 2-DG (10 mM) for 48 h, respectively. a Expression of protein was detected by Western blot. b-e Quantification of protein by densitometry of Western blot performed above. ^*p < 0.05, ^**p < 0.01 vs. control. Data were represented as mean ± SD, n = 3