Fig. 1

Effects of sotetsuflavone on A549 cells survival. a, b, c show changes of cell viability of A549 cells treated with different concentrations of sotetsuflavone for 12 h, 24 h and 48 h respectively. The viability of A549 cells were significantly different after 12 h, 24 h and 48 h compared with that of control groups (P < 0.05, ** P < 0.01, *** P < 0.001). d Molecular structure of sotetsuflavone. e The cytotoxicity of sotetsuflavone in A549 cells, there was no significant difference in IC50 values between 24 h and 48 h after drug treatment (P > 0.05). f The inhibition rate of sotetsuflavone at 12, 24 and 48 h. When the drug concentration was more than 80 μmol/L, the inhibitory effect of the three times gradients was not different (P > 0.05). Combined with Fig. 1a, b, c, e, f, the final selection of 24 h as the follow-up experimental treatment time, and the subsequent experimental concentration adjusted to 0, 64, 128 μmol/L. The results from three independent experiments were expressed as mean ± SD compared with the control group, *P < 0.01, **P < 0.01, ***P < 0.001