Fig. 4

Effect of LAC117 on PI3K/AKT signaling pathway in HCC cells. To investigate the effect of LAC117 on PI3K/AKT signaling pathway, HepG2 and Huh7 cells were treated with 100 μg/mL of LAC117 for 1 h, and expression levels of p-AKT, p-mTOR, p-GSK3β, and β-actin were determined by Western blotting analysis. Data are represented as the mean ± SEM from triplicate experiments (*P < 0.05 and **P < 0.01vs Con)