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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Puerarin inhibits vascular smooth muscle cells proliferation induced by fine particulate matter via suppressing of the p38 MAPK signaling pathway

Fig. 5

Effect of puerarin on the levels of inflammatory and oxidative stress biomarkers in VSMCs. Cells were pre-treated with puerarin at different concentrations (0, 12.5, 25, 50 μM) or p38 MAPK inhibitor SB203580 (20 μM) for 1 h and followed by the addition of 200 mg/L PM2.5 for 24 h. a IL-6 level was measured by ELISA. b TNF-α level was measured by ELISA. c SOD level was assayed by colorimetric assay kit. d MDA level was assayed by colorimetric assay kit. The results are presented as mean ± SEM. n = 3. Compared to the untreated cells, *P < 0.05, **P < 0.01; compared to 200 mg/L PM2.5 group, #P < 0.05, ##P < 0.01; compared to the co-incubation of puerarin with SB203580 group, P < 0.05, ▲▲P < 0.01

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