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Table 3 Antimutagenicity testing of the pure compounds against the four S. typhimurium tester strains

From: The mutagenic and antimutagenic activity of Sutherlandia frutescens extracts and marker compounds

Compound *conc. (%) TA97a (-S9) TA98 (+S9) TA100 (+S9) TA102 (-S9)
L-arginine 5 43.0 ± 0.9a 22.1 ± 8.4a 48.6 ± 2.5a 106.5 ± 1.0a
2.5 57.1 ± 2.0b 67.4 ± 3.7b 96.0 ± 1.1b 116.1 ± 0.7a
1 84.1 ± 1.8b 85.3 ± 3.6b 124.1 ± 2.4c 119.3 ± 0.6a
L-canavanine 5 (−) 101.9 ± 3.7a 25.1 ± 6.7a 38.9 ± 1.5a 101.1 ± 0.7a
2.5 (−) 120.6 ± 4.5a 64.8 ± 5.0b 56.7 ± 2.1b 116.2 ± 0.4a
1 (−) 138.2 ± 4.1a 80.1 ± 2.3b 92.2 ± 1.7c 125.9 ± 1.0a
D-pinitol 5 31.2 ± 8.8a 26.6 ± 7.3a 36.1 ± 1.8a 82.8 ± 3.5a
2.5 79.0 ± 6.8b 48.8 ± 6.5b 79.6 ± 1.8b 109.5 ± 4.6a
1 143.5 ± 16.6c 65.0 ± 10.3b 117.2 ± 3.0b 115.0 ± 5.3a
GABA 5 58.8 ± 3.4a 14.3 ± 7.9a 32.9 ± 2.1a 98.3 ± 1.0a
2.5 61.8 ± 2.5a 45.8 ± 5.1b 66.2 ± 2.5b 113.1 ± 0.9a
1 61.4 ± 1.4a 72.5 ± 3.0b 105.4 ± 1.5c 116.7 ± 0.4a
Positive control Revertant counts 9-AA 2-AAF AFB1 CHP
247 ± 3 358 ± 26 360 ± 3 681 ± 4
  1. Inhibition expressed as mean % inhibition ± STD of the positive control. All three mean % inhibitions of the pure compounds where compare at similar dilutions. The mM concentrations of the pure compounds (5%) were as follows: L-arginine [0.28 × 103 mM], L-canavanine [0.26x103mM], D-pinitol [0.25 × 103 mM] and GABA (gamma aminobutyric acid) [0.48x103mM]. *Note that GABA concentration is almost double that of the other three compounds. *This table also shows a dose-response increase of the antimutagenic activity of the compounds, where in most cases the highest concentration’s inhibition is more than double that of the lowest. The negative or minus (−) sign indicated co-mutagenicity of L-canavanine with 9-AA in the absence of S9 activation
  2. Abbreviations: 9-AA 9-aminoacradine, 2-AAF 2-acetamidofluorene, AFB 1 aflatoxin B1, CHP cumoyl hydroperoxide