Fig. 3

YGJDSJ activated caspases in Bel-7402 cells. After 24 h YGJDSJ (100–400 μg/ml) treatment, caspase-3 (a), caspase-3 (b) and caspase-9 (c) activities in suspension-cultured Bel-7402 cells were detected as described in Materials and Methods. Caspases activities were expressed as fold activation over control. d suspension-cultured Bel-7402 cells were pretreated with Z-VAD-FMK (50 μmol/L) for 2 h before treatment with YGJDSJ for 24 h, stained with Annexin V-FITC/PI and analyzed by flow cytometry. Data presented are from three separate experiments. *P < 0.01, versus control group; ^#P < 0.01, versus corresponding dose of YGJDSJ treated Z-VAD-FMK (−) group