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Table 3 Apoptotic lymphocytes detection by binding the surface of cells with annexin V and staining with PI under the administration of Galega officinalis extract in normal conditions and under experimental diabetes mellitus

From: Galega officinalis extract regulate the diabetes mellitus related violations of proliferation, functions and apoptosis of leukocytes

Cell viability, %  
C C + G D D + G
Viable cells
(annexin V/ PI)
96,41 ± 1,70 99,51 ± 0,07 87,56 ± 1,12* 98,12 ± 0,26#
Cells with features of early apoptosis
(annexin V+/PI)
2,75 ± 1,92 0,11 ± 0,04* 10,38 ± 0,58* 1,39 ± 0,31#
Cells with features of late apoptosis
(annexin V+/PI+)
0,06 ± 0,02 0,004 ± 0,002 0,16 ± 0,08* 0,04 ± 0,02#
Cells with features of necrosis
(annexin V/PI+)
0,78 ± 0,30 0,38 ± 0,06* 1,91 ± 1,76 0,44 ± 0,07#
  1. *P < 0.05 compared with controls. #P < 0.05 compared with diabetic rats