Fig. 4

hiPSCs have an ES cell-like property. a Representative pictures of hiPSCs line derived in the presence of SGT-4. hiPSCs lines derived from 4F reprogramming in the presence of SGT-4 were cultured in ES cells medium on Matrigel-coated plates in the absence of feeder cells. The diagram shows the comparison of the number of AP+ colonies generated from HFFs. Each value is expressed as the mean ± SD (n = 3). **P < 0.01 indicates a significant difference versus OSKM treatment alone. b RT-PCR demonstrating that three randomly selected 4F HFF-derived iPSCs express the pluripotent marker genes Nanog, c-Myc, Sox-2, Oct4, and KIf4