Fig. 2

Ex vivo effects of PM 21 on ATP release. Six male rats in each experimental group were treated with test samples. PM21 was orally administered daily after overnight fasting for 3 days to three test groups at the doses of 75 mg/kg, 150 mg/kg, and 300 mg/kg, and aspirin was administered to positive control group at the dose of 50 mg/kg. Blood was collected 1 h after the final treatment. Platelet aggregation was induced by adding 5 μg/mL collagen and terminated after 5 min. ATP release was measured with a luminometer using ATP assay kit. Data show the mean ± SEM of six measurements. **p < 0.01, ***p < 0.001 versus vehicle control