Fig. 2

Real-time RT-PCR analysis of gene expression level after treating MCF-7 cells with NDL for 24, 36 and 48 h. Fold change indicated relative quantitation normalized to GADPH. Fold change >1 and <1 indicated upregulation and downregulation, respectively. The statistical differences between the treatment and control conditions were analyzed by two-tailed paired Student’s t-tests (*p < 0.05; **p < 0.01; ***p < 0.001). a, ER and oxidative stress related genes; b, cell cyle and apoptosis related genes