Fig. 2

Gross observation and measurement of the area of hair regrowth in C57BL/6 mice. a After the dorsal skin was shaved, each mouse were orally administered the distilled water (negative control group; A-C), herbal complex = 2:1:1200 mg/kg (D-F) or herbal complex = 2:1:1400 mg/kg (G-I) group, and finasteride (1 mg/kg, as positive control group; J-L) for 25 days. Herbal complex and finasteride were dissolved in 1.0 mL of distilled water and administered through an 8 F red Rob-Nel catheter once a day. Back skin was photographed at day 0, 10, and 25 after depilation. As the duration of treatment became prolonged, hair growth was more readily visible. The experimental group (both the 200 and 400 mg/kg groups) showed darker skin than the negative control group. b The area of hair regrowth by image software. Data are presented as the mean ± SD. **p < 0.001 compared with negative control, †p < 0.05 herbal complex 200 mg/kg vs. 400 mg/kg group. Herbal complex = Houttuynia cordata Thunb, Perilla frutescens Britton var. acuta, and Green tea