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Table 4 Method validation parameters for five reference analytes

From: Pharmacological potential of Bidens pilosa L. and determination of bioactive compounds using UHPLC-QqQLIT-MS/MS and GC/MS

Parameters Analytes
Catechin Kaempferol Ferulic acid Gallic acid Paclitaxel
Regression equation 6.01× + 0.33 5.53× + 0.31 40.02× + 0.05 41.44× + 0.02 306× + 1.92
Correlation coefficient (r2) 0.9998 0.9999 0.9995 1.0000 0.9996
Linearity range (ng/mL) 1–250 1–250 0.5–100 0.1–100 5–500
LOD (ng/mL) 0.14 0.20 0.03 0.01 0.02
LOQ (ng/mL) 0.43 0.61 0.09 0.03 0.07
Precision RSD % (Intra-day, n = 6) 0.34 1.02 0.62 0.25 0.61
Precision RSD % (Inter-day, n = 6) 1.01 1.22 1.11 0.94 1.21
Stability RSD % (n = 5) 1.83 2.55 1.88 1.92 1.80
Recovery (n = 3) Mean 105.19 97.50 94.87 95.50 98.86
RSD % 0.76 1.02 1.13 1.82 0.96
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