Fig. 5From: Ethanol extract of propolis and its constituent caffeic acid phenethyl ester inhibit breast cancer cells proliferation in inflammatory microenvironment by inhibiting TLR4 signal pathway and inducing apoptosis and autophagyEECP and CAPE induced apoptosis and autophagy in LPS-stimulated MDA-MB-231 cells. a The expression of caspase 3, procaspase 3 and PARP were detected by western blotting at 24 h. b Quantification of relative expression of procaspse 3, caspase 3 and PARP. c Cells were stained with anti-LC3B antibody for immunostaining. Immunofluorescence graphs showed an increase of endogenous punctuate LC3B. d The expression of LC3B and p62 were detected by western blotting at 24 h. e Quantification of relative expression of LC3-II, LC3-II/ LC3-Iand p62 in LPS-stimulated MDA-MB-231 cells. (* P < 0.05, ** P < 0.01 vs control, n = 3). Data are means ± S.E.MBack to article page