Table 2 Antibacterial activity of the extract and fractions of the methanol stem bark extract of T. orientalis on different bacterial isolates
Test Organisms | Extract/Fraction | Concentrations (mg/mL) | |||||
|---|---|---|---|---|---|---|---|
50.000 | 25.000 | 12.500 | 6.250 | 3.125 | 1.563 | ||
Enterobacter cloaca ATCC 13047 | Ethylacetate fraction | 35.54 ± 8.34 | 18.62 ± 3.54 | 0.00 | 0.00 | 0.00 | 0.00 |
Dichloromethane fraction | 58.24 ± 12.40 | 38.50 ± 11.74 | 3.14 ± 1.01 | 0.00 | 0.00 | 0.00 | |
Methanol Extract | 102.14 ± 23.45 | 54.29 ± 10.81 | 7.07 ± 1.45 | 3.14 ± 1.32 | 0.00 | 0.00 | |
Methanol Fraction | 48.69 ± 6.01 | 50.29 ± 9.33 | 12.57 ± 4.30 | 0.00 | 0.00 | 0.00 | |
Gentamicin (10 μg/mL) | 211.13 ± 9.25 | ||||||
Escherichia coli ATCC 8739 | Ethylacetate fraction | 50.29 ± 12.37 | 56.30 ± 11.80 | 28.29 ± 3.72 | 7.07 ± 3.23 | 0.00 | 0.00 |
Dichloromethane fraction | 66.24 ± 8.26 | 48.19 ± 9.83 | 32.73 ± 2.92 | 22.29 ± 1.56 | 0.00 | 0.00 | |
Methanol Extract | 52.21 ± 9.36 | 35.50 ± 10.10 | 28.40 ± 3.30 | 12.57 ± 3.84 | 3.14 ± 0.44 | 0.00 | |
Methanol Fraction | 71.53 ± 32.23 | 44.92 ± 8.54 | 19.14 ± 1.95 | 19.34 ± 0.85 | 3.14 ± 1.93 | 0.00 | |
Gentamicin (10 μ/ml) | 144.52 ± 3.87 | ||||||
Serratiamercescens ATCC 9986 | Ethylacetate fraction | 93.07 ± 6.12 | 73.12 ± 5.27 | 78.57 ± 16.34 | 7.07 ± 2.56 | 0.00 | 0.00 |
Dichloromethane fraction | 142.09 ± 15.28 | 95.07 ± 7.23 | 78.57 ± 10.67 | 52.21 ± 3.83 | 12.57 ± 3.45 | 3.14 ± 0.45 | |
Methanol Extract | 63.6410.46± | 63.64 ± 9.34 | 0.00 | 0.00 | 0.00 | 0.00 | |
Methanol Fraction | 79.57 ± 8.10 | 96.07 ± 15.28 | 63.64 ± 3.67 | 61.45 ± 6.44 | 0.00 | 0.00 | |
Gentamicin (10 μ/ml) | 111.13 ± 3.16 | ||||||
Pseudomonas aeruginosa ATCC 19582 | Ethylacetate fraction | 64.31 ± 4.19 | 58.64 ± 7.28 | 53.29 ± 5.23 | 19.64 ± 2.45 | 3.83 ± 0.45 | 0.00 |
Dichloromethane fraction | 65.07 ± 24.34 | 28.29 ± 6.26 | 3.14 ± 0.34 | 0.00 | 0.00 | 0.00 | |
Methanol Extract | 73.57 ± 12.33 | 50.29 ± 9.28 | 38.50 ± 11.36 | 4.56 ± 1.04 | 0.00 | 0.00 | |
Methanol Fraction | 113.14 ± 23.12 | 63.64 ± 7.29 | 19.645.23 | 3.14 ± 0.77 | 0.00 | 0.00 | |
Gentamicin (10 μg/mL) | 356.50 ± 12.07 | ||||||
Enterococcus faecalis ATCC 29212 | Ethylacetate fraction | 122.79 ± 13.60 | 50.29 ± 13.92 | 19.64 | 3.36 ± 1.35 | 3.19 ± 0.31 | 0.00 |
Dichloromethane fraction | 38.50 ± 7.27 | 32.50 ± 4.65 | 12.57 | 0.00 | 0.00 | 0.00 | |
Methanol Extract | 81.81 ± 5.73 | 28.29 ± 7.12 | 19.64 | 3.14 ± 0.25 | 0.00 | 0.00 | |
Methanol Fraction | 52.19 ± 7.45 | 38.50 ± 3.71 | 38.50 | 0.00 | 0.00 | 0.00 | |
Gentamicin (10 μg/mL) | 165.20 ± 14.02 | ||||||
Staphylococcus aureus ATCC 6538 | Ethylacetate fraction | 52.19 ± 9.15 | 28.29 ± 3.27 | 29.29 ± 3.18 | 19.84 ± 3.23 | 3.18 ± 0.31 | 3.11 ± 0.23 |
Dichloromethane fraction | 53.82 ± 9.38 | 24.23 ± 3.55 | 0.00 | 0.00 | 0.00 | 0.00 | |
Methanol Extract | 50.29 ± 18.39 | 28.49 ± 7.23 | 19.64 ± 3.91 | 0.00 | 0.00 | 0.00 | |
Methanol Fraction | 53.23 ± 7.14 | 0.00 | 0.00 | 0.00 | 0.00 | 0.00 | |
Gentamicin (10 μg/mL) | 50.29 ± 4.45 | ||||||