Fig. 6

Timeline of NT2 cell differentiation with plant extract. (A1) On day 4, the morphology of NT2 cells with 5 μg/ml of RS1S CHCL3 had begun to change. (A2) On day 10 and (A3) on day 14, the NT2 cells showed short neurites that developed from day 4 until day 14. During incubation, NT2 cells were treated with 5 μg/ml of RS1S CHCL3. (B1, B2 and B3) Vehicle control (0.001% MeOH) cells maintained their morphology over the 14 days. Scale bar, 500 μm. Magnification (10×). Arrows indicate neurite extensions