Table 2 The gradient program in the positive and negative ionization mode
Lonization mode | Time(min) | Flow rate | A(%) | B(%) |
|---|---|---|---|---|
Positive Ionization mode | 0 | 300 | 90 | 10 |
2 | 300 | 30 | 70 | |
3 | 300 | 20 | 80 | |
6.5 | 300 | 0 | 100 | |
6.6 | 300 | 90 | 10 | |
8.5 | 300 | 90 | 10 | |
Negative ionization mode | 0 | 300 | 90 | 10 |
1 | 300 | 60 | 40 | |
2.2 | 300 | 40 | 60 | |
4 | 300 | 5 | 95 | |
5.5 | 300 | 5 | 95 | |
5.6 | 300 | 90 | 10 | |
8 | 300 | 90 | 10 |