Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract

Table 5 Minimum inhibitory concentrations for different fractions of Isodon rugosus against the tested bacterial strains expressed as μg/ml

Samples	P. m	S. a	E. c	B. c	S. t	K. p	P. a
Ir.Cr	6.66 ± 0.83	2.50 ± 0.00	8.33 ± 0.83	2.66 ± 1.30	12.50 ± 2.88	8.33 ± 0.83	4.16 ± 1.66
Ir.Hex	18.33 ± 0.83	16.66 ± 0.83	2.83 ± 1.16	1.33 ± 0.60	8.33 ± 2.20	10.83 ± 2.20	12.50 ± 1.44
Ir.Cf	2.50 ± 0.00	3.66 ± 1.96	1.00 ± 0.00	5.33 ± 2.60	2.01 ± 0.50	1.16 ± 0.66	2.50 ± 0.00
Ir.EtAc	7.50 ± 1.44	7.50 ± 1.44	4.16 ± 0.83	2.83 ± 1.16	3.33 ± 0.83	1.33 ± 0.60	2.83 ± 1.16
Ir.Aq	9.16 ± 0.83	11.66 ± 0.83	10.00 ± 1.44	10.83 ± 2.20	7.50 ± 1.44	5.00 ± 1.44	5.00 ± 1.44
Ir.Sp	1.00 ± 0.00	0.83 ± 0.16	5.00 ± 1.44	2.50 ± 0.00	4.50 ± 1.89	1.00 ± 0.00	3.33 ± 0.83
Ir.Flv	2.83 ± 1.16	1.00 ± 0.00	1.50 ± 0.50	6.66 ± 2.20	2.00 ± 0.50	2.66 ± 1.30	5.00 ± 1.44
Ceftriaxone	0.50 ± 0.00	0.66 ± 0.16	2.50 ± 0.00	0.83 ± 0.16	0.66 ± 0.16	0.50 ± 0.00	1.33 ± 0.60

Data is represented as mean ± SEM, (n = 3)
Keys:
P. m Protius mirabilis, S. a Streptococcus aureus, E. c Escherichia coli, B. c Bacillus cereus, S. t Salmonella typhi, K. p Klebsiella pneumonia, P. a Pseudomonas aeruginosa, E. f Enterococcus faecalis, Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Sp Saponins, Ir.Fl Flavonoids

ISSN: 2662-7671