Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract

Table 4 Antibacterial activity of Isodon rugosus against various bacterial strains expressed in mm as ZOI

Samples	P. m	S. a	E. c	B. c	S. t	K. p	P. a	E. f
Ir.Cr	17.2 ± 0.37	9.1 ± 0.34	7.2 ± 0 .55	---	28.2 ± 0.40	18.2 ± 0.35	9.3 ± 0.31	21.3 ± 0.36
Ir.Hex	07.3 ± 0.34	9.1 ± 0.52	---	7.1 ± 0.49	18.2 ± 0.37	17.2 ± 0.37	8.3 ± 0.25	23.3 ± 0.36
Ir.Cf	11.2 ± 0.32	19.2 ± 0.47	7.1 ± 0.26	---	6.1 ± 0.46	15.3 ± 0.35	9.2 ± 0.31	19.3 ± 0.39
Ir.EtAc	9.2 ± 0.40	29.3 ± 0.28	21.2 ± 0.40	17.1 ± 0.46	28.2 ± 0.35	18.2 ± 0.40	7.1 ± 0.46	8.2 ± 0.37
Ir.Aq	21.2 ± 0.31	9.2 ± 0.31	---	13.2 ± 0.20	9.2 ± 0.40	9.2 ± 0.40	17.2 ± 0.37	---
Ir.Sp	18.2 ± 0.43	27.2 ± 0.23	24.1 ± 0.52	26.3 ± 0.34	8.2 ± 0.37	21.3 ± 0.39	9.2 ± 0.40	19.3 ± 0.38
Ir.Flv	33.1 ± 0.49	16.2 ± 0.54	12.2 ± 0.54	31.3 ± 0.31	16.2 ± 0.23	27.3 ± 0.35	19.2 ± 0.38	13.2 ± 0.45
Ceft	35.2 ± 0.37	31.1 ± 0.34	9.2 ± 0.44	29.3 ± 0.42	19.2 ± 0.37	25.4 ± 0.40	36.1 ± 0.34	21.3 ± 0.41

Results are expressed as zone of inhibition (ZOI). Data is represented as mean ± SEM, (n = 3).
Keys:
P. m Proteus mirabilis, S. a Streptococcus aureus, E. c Escherichia coli, B. c Bacillus cereus, S. t Salmonella typhi, K. p Klebsiella pneumonia, P. a Pseudomonas aeruginosa, E. f Enterococcus faecalis, Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Sp Saponins, Ir.Fl Flavonoids, Ceft Ceftriaxone

ISSN: 2662-7671