Fig. 4

a DMC reduced ROS formation as stained by 1μM CM-H2 DCFDA. a Photomicrograph showing the preventive effect of DMC (50 nM) against rotenone induced ROS generation. a Control, b rotenone, c DMC + rotenone and d DMC. b ROS levels were significantly increased in rotenone (100 nM) treated cells as compared to control cells, while DMC (50 nM) pretreatment significantly decreased the levels of ROS as compared to rotenone alone treated cells. Values are given as mean ± SEM of four independent experiments in each group. *p<0.05 compared to control and #p<0.05 compared to rotenone group (DMRT)