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Table 4 Protocol for MGIT-DST for PZA R-Mtb with DFO-B alone and in combination with PZA

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

No Drug Initial Concentration of the drug preparation Volume added to MGIT for Test Final Concentration in MGIT tube except GC No. of PZA tubes
GC PZA R
1 PZA 8 mg/mL 100 μL 0.1 mg/mL 1 1
2 DFO-B + PZA 160 mg/mL + 8 mg/mL 12.5 μL + 100 μL 0.25 mg/mL and 0.1 mg/mL   1
3 DFO-B + PZA 160 mg/mL + 8 mg/mL 25 μL + 100 μL 0.5 mg/mL and 0.1 mg/mL   1
4 DFO-B + PZA 160 mg/mL + 8 mg/mL 50 μL + 100 μL 1.0 mg/mL and 0.1 mg/mL   1
5 DFO-B 160 mg/mL 12.5 μL 0.25 mg/mL 1 1
6 DFO-B 160 mg/mL 25 μL 0.5 mg/mL   1
7 DFO-B 160 mg/mL 50 μL 1.0 mg/mL   1
8 DFO-B + FeCl3 160 mg/mL + 80 mg/mL 50 μL + 50 μL 1.0 mg/mL and 0.5 mg/mL   1