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Table 3 Protocol for MGIT-DST of MDR-Mtb with DFO-B alone and in combination with INH and RIF

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

No Drug Initial Concentration of the drug preparation Volume added to MGIT for Test Final Concentration in MGIT tube except GC No. of Middle Brook tubes
GC MDR
1 INH 8.3 μg/mL 100 μL 0.1 μg/mL 1 1
2 RIF 83 μg/mL 100 μL 1.0 μg/mL   1
3 DFO-B + INH 160 mg/mL + 8.3 μg/mL 12.5 μL + 100 μL 0.25 mg/mL and 0.1 μg/mL   1
4 DFO-B + INH 160 mg/mL + 8.3 μg/mL 25 μL and 100 μL 0.5 mg/mL and 0.1 μg/mL   1
5 DFO-B + INH 160 mg/mL + 8.3 μg/mL 50 μL and 100 μL 1.0 mg/mL and 0.1 μg/mL 1 1
6 DFO-B + RIF 160 mg/mL + 83 μg/mL 12.5 μL + 100 μL 0.25 mg/mL and 1.0 μg/mL   1
7 DFO-B + RIF 160 mg/mL + 83 μg/mL 25 μL + 100 μL 0.5 mg/mL and 1.0 μg/mL   1
8 DFO-B + RIF 160 mg/mL + 83 μg/mL 50 μL + 100 μL 1.0 mg/mL and 1.0 μg/mL   1
9 DFO-B 160 mg/mL 12.5 μL 0.25 mg/mL 1 1
10 DFO-B 160 mg/mL 25 μL 0.5 mg/mL   1
11 DFO-B 160 mg/mL 50 μL 1.0 mg/mL   1
12 DFO-B + FeCl3 160 mg/mL + 80 mg/mL 50 μL + 50 μL 1.0 mg/mL and 0.5 mg/mL   1