Fig. 6From: Protective effects of aucubin on osteoarthritic chondrocyte model induced by hydrogen peroxide and mechanical stimulusGene expressions after stimulation. Gene expressions of COL2A1 (a, c), ACAN (b, d), MMP13 (e, g) and IL6 (f, h) were examined by RT-qPCR. Chondrocytes pretreated with 100 μM of aucubin for 24 h were then stimulated with 1 mM H2O2 (a, b, e and f) for 30 min, or 60 psi compression (c, d, g and h) for 24 h. Data from five independent biological replicates each with three technical replicates are expressed as fold change compared to untreated control and shown represent mean ± SD. Each target gene was normalized to GAPDH. *p < 0.05 compared with the control group. # p < 0.05 compared with the H2O2 or loading groupBack to article page