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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Protective effects of aucubin on osteoarthritic chondrocyte model induced by hydrogen peroxide and mechanical stimulus

Fig. 6

Gene expressions after stimulation. Gene expressions of COL2A1 (a, c), ACAN (b, d), MMP13 (e, g) and IL6 (f, h) were examined by RT-qPCR. Chondrocytes pretreated with 100 μM of aucubin for 24 h were then stimulated with 1 mM H2O2 (a, b, e and f) for 30 min, or 60 psi compression (c, d, g and h) for 24 h. Data from five independent biological replicates each with three technical replicates are expressed as fold change compared to untreated control and shown represent mean ± SD. Each target gene was normalized to GAPDH. *p < 0.05 compared with the control group. # p < 0.05 compared with the H2O2 or loading group

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