Fig. 6

The hexane fraction of CS reproduced the anti-inflammatory effect. Mice (5 mice per group) were sensitized on the shaved ventral abdomen on days 0 and 1 by applying 50 μl of 0.5% DNFB (positive control ■), or were treated with the vehicle alone (negative control●); all groups of mice were challenged with 20 μl of 0.2% DNFB on the right ears on day 6. Other groups of mice were sensitized and challenged with DNFB and received i.p. injections of crude extract of CS (▲) (panel a, CS), or the hexane fraction of CS (panel a, F1), the ethyl acetate fraction (panel b, F2) or the aqueous fraction (panel c, F3) (▼). Data were represented as averages of ear swelling values after the challenge. P value <0.05 was considered to be significant [* p < 0.05; ** p < 0.01; *** p < 0.001] (Additional file 6)