Fig. 5From: Anti-inflammatory potential of Capparis spinosa L. in vivo in mice through inhibition of cell infiltration and cytokine gene expressionCS inhibited cytokine gene expression in the lymph nodes draining the inflammatory site. Expression of mRNA was investigated by real-time PCR in positive control mice (sensitized and challenged with DNFB “0.5 and 0.2%”, n = 7), in negative control mice (only challenged by 0.2% of DNFB, n = 4), and in mice sensitized and challenged with DNFB “0.5 and 0.2%” and treated with CS at this two phases (Cap, n = 6). The analysis of IFNγ (a), IL-17 (b) and IL-4 (c) mRNA expression is shown. Results are represented as normalized expression: 2 − ΔCt (ΔCt = Ct RNA target – Ct β-actin). P value <0.05 was considered to be significant [* p < 0.05; ** p < 0.01; *** p < 0.001] (Additional file 5)Back to article page