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Table 2 Antinociceptive effect of D. sissoo leave extract, morphine and reversal effect of naloxone in hot plate test

From: Antinociceptive effect of methanol extract of Dalbergia sissoo leaves in mice

Treatment Dose (mg/kg) Latency period (s) (% MPE) Pretreatment 30 min 60 min 90 min 120 min
Treatment without Naloxone Control 0.1 mL/mouse 5.40 ± 1.20 7.20 ± 1.56 9.20 ± 0.86 9.60 ± 1.07 10.60 ± 1.07
Morphine 5 5.82 ± 0.86 12.60 ± 0.92* (42.19) 14.00 ± 0.70** (44.44) 15.20 ± 0.86** (53.84) 18.00 ± 1.14** (78.72)
MEDS 100 5.20 ± 0.97 8.60 ± 2.01 (10.93) 10.80 ± 0.86 (14.81) 13.00 ± 1.00 (32.69) 15.40 ± 1.28 (51.06)
MEDS 200 7.80 ± 1.46 9.40 ± 0.92 (17.18) 11.60 ± 1.20 (22.22) 14.00 ± 1.22* (42.30) 16.40 ± 1.53* (61.70)
MEDS 400 8.80 ± 1.24 11.00 ± 0.70 (29.68) 13.20 ± 1.06* (37.03) 15.20 ± 1.15** (53.84) 17.80 ± 1.53** (76.59)
Treatment with Naloxone NLX 2 7.00 ± 0.70 6.20 ± 0.58 5.20 ± 0.66 4.40 ± 0.51 4.00 ± 0.70
NLX+ Control 2 + 0.1 mL/mouse 6.64 ± 0.74 7.00 ± 0.70 7.80 ± 0.86 8.20 ± 0.86 9.00 ± 0.89
NLX+ Morphine 2 + 5 6.20 ± 0.58 8.60 ± 0.51 (10.93) 10.80 ± 0.73 (14.81) 12.20 ± 0.86 (25) 13.00 ± 1.14 (25.53)
NLX+ MEDS 2 + 100 4.80 ± 0.58 8.80 ± 0.66 (12.50) 10.60 ± 0.51 (12.96) 11.80 ± 0.80 (21.15) 15.00 ± 0.70 (46.80)
NLX+ MEDS 2 + 200 7.40 ± 1.20 9.60 ± 0.67 (18.75) 12.00 ± 0.70 (25.92) 12.40 ± 0.92 (26.92) 15.80 ± 1.59 (55.31)
NLX+ MEDS 2 + 400 8.40 ± 0.81 10.20 ± 0.58 (23.43) 12.20 ± 0.37 (27.77) 13.40 ± 0.92 (36.52) 16.60 ± 1.43 (63.82)
  1. Values are presented as mean ± SEM (n = 5). MEDS Methanolic extract of D. sissoo, NLX Naloxone
  2. **p < 0.01 compared with the control group (Dunnett’s test)
  3. *p < 0.05 compared with the control group (Dunnett’s test)