Table 2 Antinociceptive effect of D. sissoo leave extract, morphine and reversal effect of naloxone in hot plate test
From: Antinociceptive effect of methanol extract of Dalbergia sissoo leaves in mice
Treatment | Dose (mg/kg) | Latency period (s) (% MPE) Pretreatment | 30 min | 60 min | 90 min | 120 min | |
|---|---|---|---|---|---|---|---|
Treatment without Naloxone | Control | 0.1 mL/mouse | 5.40 ± 1.20 | 7.20 ± 1.56 | 9.20 ± 0.86 | 9.60 ± 1.07 | 10.60 ± 1.07 |
Morphine | 5 | 5.82 ± 0.86 | 12.60 ± 0.92* (42.19) | 14.00 ± 0.70** (44.44) | 15.20 ± 0.86** (53.84) | 18.00 ± 1.14** (78.72) | |
MEDS | 100 | 5.20 ± 0.97 | 8.60 ± 2.01 (10.93) | 10.80 ± 0.86 (14.81) | 13.00 ± 1.00 (32.69) | 15.40 ± 1.28 (51.06) | |
MEDS | 200 | 7.80 ± 1.46 | 9.40 ± 0.92 (17.18) | 11.60 ± 1.20 (22.22) | 14.00 ± 1.22* (42.30) | 16.40 ± 1.53* (61.70) | |
MEDS | 400 | 8.80 ± 1.24 | 11.00 ± 0.70 (29.68) | 13.20 ± 1.06* (37.03) | 15.20 ± 1.15** (53.84) | 17.80 ± 1.53** (76.59) | |
Treatment with Naloxone | NLX | 2 | 7.00 ± 0.70 | 6.20 ± 0.58 | 5.20 ± 0.66 | 4.40 ± 0.51 | 4.00 ± 0.70 |
NLX+ Control | 2 + 0.1 mL/mouse | 6.64 ± 0.74 | 7.00 ± 0.70 | 7.80 ± 0.86 | 8.20 ± 0.86 | 9.00 ± 0.89 | |
NLX+ Morphine | 2 + 5 | 6.20 ± 0.58 | 8.60 ± 0.51 (10.93) | 10.80 ± 0.73 (14.81) | 12.20 ± 0.86 (25) | 13.00 ± 1.14 (25.53) | |
NLX+ MEDS | 2 + 100 | 4.80 ± 0.58 | 8.80 ± 0.66 (12.50) | 10.60 ± 0.51 (12.96) | 11.80 ± 0.80 (21.15) | 15.00 ± 0.70 (46.80) | |
NLX+ MEDS | 2 + 200 | 7.40 ± 1.20 | 9.60 ± 0.67 (18.75) | 12.00 ± 0.70 (25.92) | 12.40 ± 0.92 (26.92) | 15.80 ± 1.59 (55.31) | |
NLX+ MEDS | 2 + 400 | 8.40 ± 0.81 | 10.20 ± 0.58 (23.43) | 12.20 ± 0.37 (27.77) | 13.40 ± 0.92 (36.52) | 16.60 ± 1.43 (63.82) | |