Fig. 5

Neuroprotective effects of WLR in the DRGs of OXIPN animals. a Induction of neuropathy by oxaliplatin (L-OHP) and drug administration were done as described in Fig. 2. Four weeks after administration of WLR (250 mg/kg, 6 times a week) or amifostine (Ami, 100 mg/kg, once a week), DRG neurons from L4-L5 were procured and subjected to Azan-Mallory staining. The nuclei were observed at a high magnification (X400), and DRG neurons with nucleus containing atypical nucleoli were scored. When compared with the saline control mice, the DRGs of oxaliplatin (L-OHP)-treated mice showed an increased number of DRG neurons with eccentric nucleoli (arrowhead) and/or multinucleolated nuclei (arrows). b The neurons with multiple or eccentric nucleoli were counted and expressed as a percentage of the total neuron. *p < 0.05 vs saline control mice (Ctrl); ^# p < 0.05 vs. oxaliplatin (L-OHP) and vehicle (Veh)-treated mice. The data represent the combined results of n = 5 experiments