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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Essential oils from two Allium species exert effects on cell proliferation and neuroblast differentiation in the mouse dentate gyrus by modulating brain-derived neurotrophic factor and acetylcholinesterase

Fig. 3

Immunohistochemistry for doublecortin (DCX) in the dentate gyrus in vehicle-treated (vehicle, a, and b), garlic essential oil-treated (garlic oil, c, and d) and chives essential oil-treated (chives oil, e, and f) groups. DCX-immunoreactive (+) neuroblasts are seen in the subgranular zone of the dentate gyrus and their dendrites are observed in the molecular layer (ML) of the dentate gyrus. Note that DCX+ neuroblasts and their dendrites are abundant in the garlic essential oil-treated group, while in the chives essential oil-treated group, DCX+ neuroblasts and their dendrites are few. GCL, granule cell layer; ML, molecular layer; PL, polymorphic layer. Scale bar = 50 μm (a, c, e), 25 μm (b, d, f). g Number of DCX+ neuroblasts per section for each group (n = 5 per group; *p < 0.05, versus vehicle group). Data are presented as mean ± SEM

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