Fig. 8

Induction of early and late apoptosis by chalepin at various concentrations in A549 cell line. a shows the flow cytometric density plot of Annexin V-FITC/PI staining in A549 cells when treated with different concentrations of chalepin (18.0-45.0 μg/ml) at 48 h incubation. b Shows the flow cytometric density plot of Annexin V-FITC/PI staining in A549 cells when treated with different concentrations (18.0 - 45.0 μg/ml) at 72 h incubation time. c The bar chart shows the total annexin V positive cells. d The bar charts shows the distribution of cells in viable, early apoptosis, late apoptosis and secondary necrosis phase in 48 h incubation. e The bar charts shows the percentage of distribution of the treated cell population in viable, early apoptosis, late apoptosis or secondary necrosis phase in 72 h incubation. Doxorubicin served as the positive control. The data is expressed as mean ± S.D. from three replicates. Asterisks indicates significantly different value as compared to control (*p < 0.05)