Fig. 10
a Dot plots obtained from flow cytometer shows the attenuation of mitochondrial membrane potential (MMP) in A549 cells treated with chalepin at various concentrations and incubated at 48 h. b Bar chart represents the percentage of JC-1 that remained as green monomer which shows the apoptotic cells with compromised mitochondrial membrane potential upon treatment of chalepin in dose dependent manner. The data expressed as mean ± S.D from triplicates in experiment. Asterisks indicates significantly different value from control (*p < 0.05). c Effects of chalepin at different concentrations on mitochondrial membrane potential changes of A549 cell line at 48 h and 72 h treatment time. After the incubation period, the cells were stained with JC-1 dye and were examined with a fluorescent microscope under magnification of 630x to observe whether the JC-1 aggregates in mitochondria (healthy cells) or JC-1 remains in monomer form at cytoplasm (apoptotic cells)