Fig. 6

Inhibitory effects of WSR on STAT-1 and Jak-2 phosphorylation in TNF-α/IFN-γ-stimulated HaCaT cells. HaCaT cells were pre-treated with the indicated concentrations of WSR for 2 h and then stimulated with TNF-α/IFN-γ (each 10 ng/mL) for 30 min. a The phosphorylation or protein level in whole-cell lysates was determined by Western blotting using antibodies against phospho-STAT-1, STAT, and the band density of p-STAT-1/STA-1. b The phosphorylation or level of each protein in whole-cell lysates was determined by Western blotting using antibodies against phosphor-Jak-2, Jak-2, and the band density of p-Jak-2/Jak-2. Data are presented as the means ± SEM of three experiments. Significance *p < 0.05, **p < 0.01, ***p < 0.001. The English in this document has been checked by at least two professional editors, both native speakers of English. For a certificate, please see: http://www.textcheck.com/certificate/6AbvJG