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Fig. 1 | BMC Complementary and Alternative Medicine

Fig. 1

From: A water extract of Malva verticillata seeds suppresses osteoclastogenesis and bone resorption stimulated by RANK ligand

Fig. 1

WEMV has a negative effect on osteoclastogenesis. BMMs were cultured for 4 days in differentiation medium containing WEMV at concentrations of 20, 40, 80, and 160 μg/mL. a TRAP-positive stained cells were visualized using TRAP staining solution. b TRAP activity was measured by TRAP assay with p-nitrophenyl phosphate as described in the Methods. c TRAP-positive stained MNCs were counted under an inverted microscope (40× magnification). d Cell viability percentage were determined using a CCK assay after a 2-day incubation with the indicated concentrations of WEMV. e BMMs were cultured in differentiation medium with WEMV (160 μg/mL) for the indicated numbers of days. TRAP-positive stained MNCs were counted on day 4. **p < 0.01 versus control

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