Skip to main content

Table 1 Zone of inhibition (in mm) of the different concentrations of crude extract and solvent fractions of the leaves of Rhamnus prinoides against gram positive bacteria

From: Evaluation of the in vitro antibacterial activity of the solvent fractions of the leaves of Rhamnus prinoides L’Herit (Rhamnaceae) against pathogenic bacteria

Category of test Concentration Bacteria
S. aureus S. pyogen S. pneumoniae
Stand Clinic Stand Clinic Stand Clinic
Crude 19.5 mg/well 16.67 ± 0.88a3d2 16.67 ± 0.33a3c1d3 13.67 ± 0.88a3c2d3 12.33 ± 0.33a3c1d2 13.33 ± 0.33a3d2 11.67 ± 0.33a3c2d3
39 mg/well 19.67 ± 0.88a3 19.00 ± 0.58a3d1 18.33 ± 0.67a3 16.33 ± 0.67a3 16.00 ± 1.00a2 15.33 ± 0.33a3d2
78 mg/well 22.00 ± 0.58a2 21.67 ± 0.33a2 20.33 ± 0.33a3 18.67 ± 0.33a3 19.33 ± 1.20a1 18.00 ± 0.00a3
Cef 0.03 mg/disc* 28.33 ± 0.58 26.33 ± 0.67 NT NT NT NT
Amp 0.01 mg/disc* NT NT 27.33 ± 0.67 26.33 ± 1.20 24.00 ± 0.58 23.00 ± 0.58
Methanol 19.5 mg/well 13.00 ± 0.58a3d2e2 11.67 ± 0.33 a3d2e3 12.67 ± 0.33a3d1 11.67 ± 0.33a3d2 12.33 ± 0.88a3d1 11.33 ± 0.33a3d3
39 mg/well 14.33 ± 0.33a3f3 13.33 ± 0.33a3f3 14.00 ± 0.58a3f2 12.67 ± 0.33a3d1f3 14.33 ± 0.33a3 13.67 ± 0.33a3d1
78 mg/well 16.17 ± 0.44a3g3 15.33 ± 0.67a3g3 15.00 ± 0.58a3g3 15.00 ± 0.58a3g3 16.00 ± 0.58a3 15.33 ± 0.33a3g2
Cef 0.03 mg/disc* 27.67 ± 0.33 26.33 ± 0 .33 NT NT NT NT
Amp 0.01 mg/disc* NT NT 28.00 ± 0.00 26.33 ± 0 .67 25.00 ± 0 .58 23.67 ± 0.33
Chloroform 19.5 mg/well 11.67 ± 0.33a3d1e3 11.67 ± 0.33a3d1e3 12.00 ± 0.58 a3d2 10.67 ± 0.33a3d2 12.00 ± 0.58a3d2 11.00 ± 0.00a3d2
39 mg/well 13.00 ± 0.58a3f3 13.33 ± 0.33a3f3 13.67 ± 0.33 a3f3 12.00 ± 0.58 a3f3 14.00 ± 0.58a3 13.00 ± 0.58a3f2
78 mg/well 14.33 ± 0.33a3g3 14.00 ± 0.58a3g3 15.00 ± 0.58 a3g3 13.67 ± 0.58a3g3 15.67 ± 0.33a3g1 15.00 ± 0.58a3g3
Cef 0.03 mg/disc* 27.00 ± 0.57 26.33 ± 0.33 NT NT NT NT
Amp 0.01 mg/disc* NT NT 27.67 ± 0.33 27.00 ± 0.58 25.00 ± 0.58 23.67 ± 0.33
  1. Values are expressed as Mean ± S.E.M (n = 3), analysis was performed with One-Way ANOVA followed by Tukey test; acompared to positive control, bto 19.5 mg/well, cto 39 mg/well, dto 78 mg/well, eto crude 19.5 mg/well, fto crude 39 mg/well, gto crude 78 mg/well; 1P < 0.05, 2P < 0.01, 3P < 0.001. The negative control has shown no antibacterial activity. * = positive controls, Stand = standard (ATCC) strains, Clinic. = clinically isolated strains, NT = not tested, Cef = Cefoxitin, Amp = ampicillin, Cip = Ciprofloxacin