Fig. 4

SK-BR-3 cell cycle analysis of cells treated with (a) VAE (b) Trastuzumab (c) 1 μg/ml Trastuzumab and VAE and (d) 10 μg/ml Trastuzumab and VAE. Cells were harvested after 3d, fixed, stained and analyzed for DNA content by flow cytometry. The distribution and percentage of cells in G0/G1, S and G2/M phase of the cell cycle are indicated. Results are presented as mean values from three independent experiments. (SE values are omitted for clarity). Significance values are given relative to the VAE and Trastuzumab untreated controls (**p < 0.01, ***p < 0.001). Discrepancies from 100 % can be attributed to slight gating differences and the display of mean values