Fig. 4

Plasmatic levels of ions of rats treated with Roundup and/or Dig1. Sodium levels in mmol/L (a) were determined via an enzymatic test of sodium dependent β-galactosidase activity with o-nitrophenyl-β-D-galactopyranoside as substrate. Potassium levels in mmol/L (b) were determined by dosing potassium-dependent pyruvate kinase activity using phosphoenolpyruvate as substrate. Inorganic phosphate in mg/L (c) was measured by a direct method quantifying unreduced phosphomolybdate heteropolyacid at 340 nm. Significant differences in scatter plots (with mean ± SEM) were reported: p < 0.05 (*), p < 0.01 (**) and p < 0.001 (***)