Fig. 2

PPHs regulate Tg-induced ER stress response in MC3T3-E1 osteoblastic cells. a MC3T3-E1 cells were treated with 0.1 μM Tg in the presence or absence of 25, 50, or 100 μg/mL PPHs for 24 h. Immunoblotting was performed using antibodies against GRP78, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, p-eIF2α, eIF2α, ATF6α, and β-actin. b Cells were treated with 0.1 μM Tg in the presence or absence of 100 μg/mL PPHs for 0, 12, 24, or 48 h. Immunoblotting was performed with antibodies against GRP78, CHOP, p-PERK, PERK, p-IRE1α, IRE1α, p-eIF2α, eIF2α, ATF6α, and β-actin. ^*p < 0.05, significantly different from the Tg-treated condition. Tg, thapsigargin; PPHs, porcine placenta hydrolysates