Fig. 3

Effect of VYE on (a) iNOS, COX-2 protein levels, and (b) HO-1 protein induction in RAW 264.7 cells. Cells were treated with (a) LPS alone or LPS plus VYE for 24 h or with (b) VYE alone for 6 h. Protein levels were determined by Western blotting, as described in the Materials and Methods, and quantitated using a Davinch-chemi™ CAS-400SM chemiluminescence imaging system (Core Bio, Seoul, Korea). β-actin served as a control. The experiment was repeated three times independently, and similar results were obtained