Fig. 3

Huh-7 cells were cultured in 6-well plates with different concentrations of MP. a The cells were stained with 10 μM DCF-DA and kept in dark for 60 min. The cells were resuspended in PBS-EDTA solution and flow cytometry was performed to determine the ROS release. (i) untreated control cells, and (ii) 10 μg/ml, (iii) 20 μg/ml, (iv) 50 μg/ml, (v) 100 μg/ml, (vi) 200 μg/ml and (vii) 500 μg/ml. b Nitric oxide release was measured in terms of nitrite production using Griess reagent. After the incubation with different concentrations of MP, Griess reagent was added to the medium and the nitrite formation was measured using Nitrate/nitrite colorimetric assay kit (Cayman). In parallel experiments, curcumin (50 μM) was used as a positive control (n = 3; ^# p < 0.05 and ^## p < 0.01)