Fig. 8

Effect of mammea E/BB on p-ERK1/2 in K562 cells. a K562 cells were treated with 3.5 μM mammea E/BB for 72 h using a human phospho-kinase array kit. Cells were treated with various concentrations for 72 h. b The p-ERK1/2 protein levels of K562 cells were detected by Western blotting. The levels of WT1 protein expression were assessed by immunoblotting; GAPDH was used as a loading control. c The protein levels were analyzed with a scan densitometer and the graphs were plotted as the percentage of vehicle control (0.003 % DMSO alone without the mammea E/BB in the culture medium). Data are the mean value ± SEM of three independent experiments. Asterisks (*) denote values that were significantly different from the vehicle control (p < 0.05)