Fig. 4
Mammea E/BB treatment induced S phase cell cycle arrest in K562 cells. a Cell cycle analysis profile after incubation with 3.5 μmol/L mammea E/BB for 72 h, using flow cytometry. b Percentage of sub G1, G0/G1, S, and G2M cell populations of the K562 cells after treatment with mammea E/BB for 72 h are presented and compared to the vehicle control group (VC). c Cyclin B, cyclin A, and cdc2 protein levels in K562 cells treated with various concentrations (1.5, 2.5, and 3.5 μM) of mammea E/BB for 72 h, detected by Western blotting. GAPDH was used as a loading control. d The protein levels presented as the percentage of vehicle control (0.006 % DMSO alone without the mammea E/BB in the culture medium), analyzed with a scanning densitometer. The percentages of cells in each phase and protein levels are represented as mean ± SEM of three independent experiments. Asterisks (*) denote values that were significantly different from the vehicle control (p < 0.05)