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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Rhus verniciflua Stokes (RVS) and butein induce apoptosis of paclitaxel-resistant SKOV-3/PAX ovarian cancer cells through inhibition of AKT phosphorylation

Fig. 3

The effect of apoptosis in butein-treated SKOV-3/PAX cells. (a) The effect of butein, fisetin, and quercetin on the viability in SKOV-3/PAX cells for 72 hours was determined by MTT assay. (b) The cytotoxic effect of butein in SKOV-3/PAX cells for 24 hours was determined by PI staining assay. Bottom panel, data represent quantitative results for top panel (mean ± SD; n = 3). *, p < 0.05 versus control. (c) The dead cells were differentiated between apoptosis and necrosis by Annexin V- FITC and 7-AAD dual staining. (d) The expression of procaspase-9, cleaved caspased-9, cleaved caspase-8, procaspase-3, caspase-3, and PARP in cell lysates from SKOV-3/PAX cells treated with butein were determined by Western blot assay

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