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Table 1 Preliminary qualitative phytochemical analysis of Passiflora incarnata methanolic extract (PI-ME)

From: Passiflora incarnata attenuation of neuropathic allodynia and vulvodynia apropos GABA-ergic and opioidergic antinociceptive and behavioural mechanisms

Sample Test Observation Result
1. Aqueous solution of PI-ME + 10 % ammonium hydroxide solution Appearance of yellow coloration Flavonoids present
2. A portion of PI-ME + few drops of Wagner’s reagent Reddish brown precipitate Alkaloids present
3. A small volume of PI-ME + 1–2 drops of Mayer’s reagent Creamy or white precipitate Alkaloids present
4. 0.5 ml PI-ME + 0.5 ml benedict’s reagent → mixed and boiled for 2 min Characteristic colored precipitate Carbohydrates present
5. 1 ml PI-ME + 1 ml Barfoed’s reagent → boiled for 2 min Red precipitate Carbohydrates present
6. 50 mg PI-ME + 5 ml distilled water + small amount of 5 % ferric chloride solution Intense green coloration Tannins and phenolic compounds present
7. 50 mg PI-ME + conc. HCL → heated on water bath for 2 h → resultant hydrolysate filtered → 2 ml hydrolysate + 3 ml chloroform → chloroform layer separated out + 10 % ammonia solution Pink coloration Glycosides present
8. A small amount of PI-ME → compressed between two pieces of filter paper Formation of oil spot on filter paper Fixed oils present
9. 50 mg PI-ME + 20 ml distilled water → shaken for 15 min Formation of 2 cm thick layer of foam Saponins present